FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose2.5_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose2.5_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4161726
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA100010.2403089487390568No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC51320.1233142210707769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG42000.067.0818256
CTATACC40200.065.819426
GTATAGG112950.065.72236
TTATAGC69200.065.497086
CTATAGG60050.065.452326
TTATAGG106950.065.3842246
CCTATAC31700.065.3613055
GGTATAC55650.065.344445
CTTATAG54150.065.1508945
TTATAAG68850.064.5083546
CTATAAC40450.064.374336
GGTATAG96550.064.3072055
TATACCC53900.064.284267
GCTATAC45750.064.260855
CGTATAA48300.064.128995
TATACGG64550.063.817317
AGTATAG75150.063.804295
CGTATAC42150.063.770815
TATAGCG68300.063.7467357
CGTATAG57100.063.746385
Produced by FastQC (version 0.11.5)