FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose1_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose1_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5204706
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA135330.2600146867085288No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC67140.1289986408454195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG55650.067.1038066
CCTATAC39150.066.867915
CTATAGG74000.066.118556
CTATACC50300.066.100316
CTTATAG71600.065.793045
GGTATAC68550.065.554955
TTATAGG131300.065.332086
TTATAGC82600.065.208846
GCTATAC56200.065.014785
CGTATAG69900.064.739495
TATAGTC96100.064.533957
TATAGCG84100.064.503557
GTATAGG134200.064.4157946
CTATAAC52450.064.191496
CCTATAG46100.063.923285
CGTATAA62750.063.9173555
AGTATAG90050.063.506225
TATACGG86400.063.353547
TTTATAG116050.063.271525
TTATAAG90450.063.2640656
Produced by FastQC (version 0.11.5)