FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4341684
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG37700.065.63526
CCTATAC33450.064.453035
GTATAGG105250.064.0461356
GGTATAC55100.063.9641465
CTATAGG53250.063.7545056
CTATACC35750.063.34136
TTATAGG100150.063.253766
GGTATAG96800.062.7672545
TATACGG60500.062.7094047
CGTATAG56300.062.41435
GCTATAC48250.062.3820885
CTATAAC40450.062.038236
TATAGTC71200.061.9860347
TTATAGC69500.061.84046
TATAGGG99600.061.6703727
CGCTATA43700.061.268714
TATAGCG65500.061.2353327
CTTATAG56000.061.1861955
TATAGGC78600.061.1818927
TTTATAG104250.061.101815
Produced by FastQC (version 0.11.5)