FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4833540
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA125480.25960269285037463No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC85650.17719931975322434TruSeq Adapter, Index 4 (100% over 50bp)
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC65020.134518386110387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG53100.067.625076
CTATAGG70900.066.8879556
CTATACC50800.066.346416
CTTATAG67450.066.158115
CCTATAC40700.065.870245
TTATAGC79900.065.442326
CGTATAG63700.065.2178045
GCTATAC53950.065.197295
TTATAGG119000.065.0862666
TATAGCG82900.064.931697
CTATAAC48950.064.9214256
CGTATAA53900.064.8681645
CCTATAG45700.064.560415
GTATAGG124000.064.494016
TATACGG77050.064.1836557
TTATAAG80400.064.164736
GGTATAC59850.064.15015
AGTATAG84000.064.123065
TATAGTC90600.064.087467
TATAACC51300.063.857727
Produced by FastQC (version 0.11.5)