FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose10_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose10_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3371549
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA74010.21951334534957076No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC62350.18492983492157464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG33350.067.691176
CTATAGG54700.066.928726
CCTATAC25750.066.601955
TTATAGC52100.066.506746
CGTATAG48850.066.131035
GGTATAC49700.065.9155045
CTATACC31450.065.771076
GCTATAC35250.065.730515
TTATAGG96500.065.430066
CTTATAG48200.065.28015
GTATAGG100300.065.254256
TTATAAG59250.064.9198466
TATAGCG55600.064.712247
CCTATAG35800.064.622925
AGTATAG70900.064.47115
CTATAAC34500.064.318856
TATACGG59600.064.30377
TATAGTC70500.064.2907947
CGTATAA39950.064.130185
GTATAAG63900.064.084526
Produced by FastQC (version 0.11.5)