FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3729014
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC101010.2708758937349122TruSeq Adapter, Index 9 (100% over 50bp)
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA96410.2585401931985238No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC73380.1967812402956921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG62650.067.3173146
CTATACG38300.067.257366
GGTATAC51750.066.3467255
TTATAGG100900.066.044496
TTATAAG62950.065.995736
CGTATAG50000.065.93895
CTATACC35900.065.90426
CTATAAC36900.065.825466
GCTATAC40150.065.7274255
CCTATAC30650.065.659595
TTATAGC57150.065.344496
TATAGCG61000.065.293997
TATACGG60250.065.177347
GATATAC40300.065.135395
CTTATAG50700.065.0975345
GTATAGG99900.064.8838046
CGTATAA44000.064.828475
GTATAAG74300.064.487496
AGTATAG73750.064.446395
TATACGC40550.064.3885657
Produced by FastQC (version 0.11.5)