FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose10_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose10_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032319
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA84550.278829503096475No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC53880.17768579097383883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACC29600.066.9227756
CCCGGTA2250.066.8859942
CGTATAG42050.066.667745
CCTATAC23100.066.3607565
TTATAGC44350.066.2879946
CTATACG31700.066.243186
GTATAGG86050.066.092436
TTATAGG79500.065.858796
CTATAGG47000.065.752476
CTTATAG42600.065.4783865
TATAGCG50550.065.081257
GGTATAG77400.065.0230265
GGTATAC43200.064.974045
TTATAAG54050.064.622546
CGTATAA36850.064.583365
TTTATAG71500.064.465745
TATAAGG60050.064.460157
AGTATAG61050.064.378875
TATAGCC31200.064.2760547
GTATAGC46000.064.214616
Produced by FastQC (version 0.11.5)