FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose0.25_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose0.25_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4400234
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA130690.297006931904076No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC70630.16051419083621463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG65150.067.09756
CTATACG45350.066.91146
CCTATAC38300.066.8000645
CTTATAG59300.066.516225
CTATACC44350.066.447226
CGTATAG59900.065.8499455
TTATAGG116800.065.533646
TTATAGC72600.065.515066
GCTATAC48450.065.375215
CGTATAA49450.065.256375
CTATAAC41750.064.884776
GGTATAC59950.064.685795
TATAGCG74300.064.675547
GTATAGG114000.064.595046
CCTATAG40600.064.567515
TTATATC73300.064.411936
TTATAAC58650.064.389016
TATACGG71450.064.365257
TATAGTC89650.064.2986157
TATACCC64500.064.246627
Produced by FastQC (version 0.11.5)