FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose0.25_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose0.25_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4385330
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA125830.28693393655665594No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC83250.18983748087373128No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT79250.18071616047138983TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG42150.068.005556
CTATAGG65200.067.5291066
GCTATAC44900.066.724525
TTATAGC66400.066.4668356
CTATACC43400.066.369436
CCTATAC34000.066.08675
CTTATAG57150.066.0789645
GGTATAC56650.065.920815
TTATAGG108750.065.524926
CGTATAA51900.065.2104345
TTATAAG76750.065.210226
CTATAAC43450.065.08486
GTATAGG114650.064.9003756
TATAGCG72800.064.806197
AGTATAG79100.064.733025
CGTATAG61350.064.5217365
TATAGCC46200.064.3166967
TATAGGC81950.064.275517
CGCTATA40200.063.9039964
TATACGC49750.063.8779227
Produced by FastQC (version 0.11.5)