FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose0.25_rep1w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose0.25_rep1w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3999034
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGGA75400.18854553374639976No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC41880.1047252911578146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAC47500.069.114466
CGTATAA56000.069.0611655
TTATAGG105650.068.9716956
CTATAGG75550.068.933156
TCTATAG69850.068.896315
CTATACG43650.068.795926
AGTATAG77450.068.6881565
TTATACC69350.068.686496
CTATACC55100.068.6647346
GTATAGG102950.068.6387946
CCTATAC38150.068.622535
TTATAAC51650.068.575646
CTTATAG72300.068.5463945
CTATAGC65000.068.544836
GCTATAC50450.068.5417945
GTATAGC69200.068.531926
CGTATAG61900.068.5285645
GGTATAC52050.068.519335
TTATAAG64200.068.47226
GTATAAC48100.068.470616
Produced by FastQC (version 0.11.5)