FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmMesophyll_dose0.25_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmMesophyll_dose0.25_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5288112
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAGTATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCC143380.2711364660960282No Hit
TAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTG129950.2457398784292012No Hit
TAAGCATATATCCGGAGATTCCCAAATAGGTCAACCTTTTGAACTGCCTG73780.13952049427092317No Hit
TGAAAATAAGCATAGATCCGGAGATTCCCAAATAGGTCAACCTTTTGAAC70530.13337463351759568No Hit
ACCTACTATAAATACTCCGCAATTATGTGCGGATTCTAAAATTAGTGGAG62790.1187380297542866No Hit
TTCCCATATAGGTCAACCTTTTGAACTGCCTGCTGAATCCATGAGCAGGC56650.10712708051569256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG117850.068.810776
TTATAGG187800.068.600956
CTTATAG88200.068.332065
CTATACG36800.068.286776
CTATACC40150.068.168096
TTATAAG123600.068.073176
GCTATAG112700.068.0111545
AATATAG229100.067.905775
GTATAAG122050.067.675916
CCTATAC29450.067.621835
GTATAGG202150.067.557496
CCTATAG59500.067.528155
CTATAAC33900.067.520876
TTTATAG125250.067.427895
TTATAGC82650.067.330876
TCTATAG90150.067.319885
AGTATAG237100.067.282595
CGTATAA50400.067.151535
ATTATAG141400.066.978955
CTATAGA109000.066.9482966
Produced by FastQC (version 0.11.5)