FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_5mM-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_5mM-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4390748
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC66810.15216086188503644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG40650.066.812086
TTATAGG96850.066.420136
GTATAGG103250.066.404616
CTATAGG54350.066.262786
CCTATAC33750.065.43495
GGTATAG95850.064.995265
GGTATAC52150.064.830095
TATAGGG99550.064.7944647
CTTATAG54600.064.741485
CGTATAG55500.064.5114065
TTATACC48600.064.380616
AGTATAG69150.064.0765
GCTATAC48150.063.8193055
CTATAAC40350.063.579096
TTATAGC65150.063.5512586
CTATACC36200.063.3266566
GTTATAG98950.063.029775
CTTATAC56550.063.004135
GTATAGC61550.062.9467666
TATAGCG63350.062.760387
Produced by FastQC (version 0.11.5)