FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_5mM-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_5mM-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4191891
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC55440.13225534728837177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG94000.066.796096
CTTATAG49750.066.3399355
TTATAGG86650.066.322526
CTATAGG52300.065.84916
CTATACG39900.065.76
CGTATAG49850.065.294145
GGTATAC52050.064.753315
CTATAAC39050.064.7101756
CTATACC34050.064.447626
TATAGGG95400.064.385067
CCTATAC31750.064.2665
TTATAGC62250.064.263346
GGTATAG90600.064.0490655
GCTATAC46850.063.7976535
AGTATAG69400.063.6942635
TATAGCG60100.063.0681957
GATATAC46650.062.9457975
TTATAAC47850.062.8300826
GATATAG68000.062.6895035
CGTATAC42600.062.4396445
Produced by FastQC (version 0.11.5)