FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_5mM-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_5mM-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4219057
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC80490.19077722818155812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG100600.066.8676
TTATAGG97000.066.606476
CTATACG39350.066.529366
CGTATAG49500.066.2507555
CTTATAG51400.065.776455
GGTATAC55500.065.583835
CTATAGG54850.064.893436
GATATAC46300.064.706695
CCTATAC29500.064.659295
TATAGGG98800.064.5073857
GGTATAG94500.064.294585
AGTATAG69300.064.0891955
CTATACC33200.063.989256
TTATAGC62350.063.935746
TATAGGC71850.063.7143757
TATAGCG60650.063.7081077
GCTATAC43900.063.6201675
TATAGGT103300.063.1541677
TATACGG64050.063.003787
CGTATAC42750.062.8755155
Produced by FastQC (version 0.11.5)