FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_2.5mM-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_2.5mM-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4402255
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG33000.065.438576
CTATAGG45300.065.2088856
GTATAGG97550.064.4020546
GGTATAG89300.064.3552865
TTATAGG91350.063.7539836
GGTATAC51350.063.3195535
CTTATAG51850.062.573945
GTTATAG103400.062.0108225
CCTATAC35250.061.8573725
CTATAAC35050.061.4114886
CGTATAG48700.061.231265
CTATACC32450.061.1548466
CCTATAG32600.061.0881845
TTATAGT126600.059.9084056
TTATAGC67100.059.7756966
TATAGGG91000.059.6915557
GCTATAC47100.059.6701555
GTATAGC61400.059.567656
AGTATAG68650.059.5476845
TATACCC36800.059.4421847
Produced by FastQC (version 0.11.5)