FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_2.5mM-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_2.5mM-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7054435
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG164200.066.8433766
TTATAGG154300.066.7090156
CTATACG66450.066.574446
CTTATAG82900.066.240545
CTATAGG88550.065.492186
CGTATAG88400.065.009435
CTATACC60400.064.898786
GGTATAC91750.064.428655
TATAGGG154500.064.334717
CCTATAC58050.064.3306055
TTATAGC105950.064.151056
GGTATAG154950.064.057525
AGTATAG114700.064.017335
GATATAG109100.063.966985
GCTATAC77900.063.708045
TATAGGC114650.063.5568167
TATAGCG102550.063.274627
CTATAAC71700.063.261776
TTATAAC87450.063.1142436
GTTATAG165000.063.04065
Produced by FastQC (version 0.11.5)