FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_2.5mM-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_2.5mM-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3205585
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC64060.19983871898577016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG74600.067.698456
CTATAGG43850.067.283596
GGTATAC39350.067.06225
TTATAGG70650.066.826856
CTATACG36950.066.492696
CTATACC33100.065.979316
CTATAAC33500.065.922826
CTTATAG37100.065.8465045
GCTATAC39600.065.843415
TTATAGC47650.065.663776
CGTATAG42350.065.6172945
CCTATAC27850.065.598895
AGTATAG52700.065.481335
TATAGCG50550.065.2200247
GGTATAG70250.065.1647345
TATAGGG75800.065.103027
TATAGGC55450.064.8216557
GTATAGA46500.064.653416
CCTATAA31650.064.025955
CGTATAC36700.063.989355
Produced by FastQC (version 0.11.5)