FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_1mM-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_1mM-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3903969
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC81990.21001703650823048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG35600.066.655576
GTATAGG95750.066.6353456
TTATAGG88950.066.417616
CTATACC34800.066.176446
CGTATAG49400.066.172375
CTTATAG49250.065.663275
CTATAGG51500.065.308986
GGTATAG89350.065.023495
TATAGGG96200.064.868387
GGTATAC49600.064.423735
CGTATAC39900.064.38435
CTATAAC39250.064.380496
TTATAGC62600.064.127726
AGTATAG66150.064.125325
CCTATAC29300.064.025645
TATAGCG61350.064.008127
GTATAGC56850.063.8417936
GCTATAC42550.063.8291325
TATAGTC63000.063.720577
GATATAG63350.063.6447565
Produced by FastQC (version 0.11.5)