FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_1mM-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_1mM-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3133895
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC43590.13909208827992003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG74250.066.9810266
CTATACG29400.066.7835856
CGTATAG37600.065.250585
CTTATAG37900.064.826435
TTATAGG72950.064.672376
GGTATAG69200.064.6366655
GGTATAC37500.064.491285
CTATAAC26350.064.286376
CTATAGG39000.064.074886
TATAGGG74450.063.98057
CCTATAC23650.063.9303055
CTATACC24150.063.766086
AGTATAG53150.063.6762355
GCTATAC33200.063.356415
TTATAGC45500.063.2287526
GGTATAA61000.062.5963675
GTATAGA47550.062.5637256
TATAGGC52250.062.5625957
TTATAGT75200.062.3650366
TATAGCG44750.062.3332067
Produced by FastQC (version 0.11.5)