FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_1mM-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_1mM-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775633
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC66620.2400173221747976No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA29750.10718275795106919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG67200.067.029016
CGTATAG35000.066.897765
CTATACG26550.066.70216
GGTATAC35600.066.6550755
TTATAGG64500.066.633436
CTATAGG37500.066.171126
CTTATAG34350.066.125885
CCTATAC20950.065.988245
TTATAGC41600.065.791076
CTATAAC28850.065.1451346
GATATAC31400.064.98195
CTATACC26450.064.969476
TTATAAC36900.064.496486
CGTATAC29150.064.4746865
GCTATAC32500.064.290155
GGTATAG61950.064.235145
TATAGGG71900.064.15647
TTTATAG57150.064.057345
TATAGGC49000.063.9978567
ACTATAC31900.063.743955
Produced by FastQC (version 0.11.5)