FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_10mM-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_10mM-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3236255
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC62780.19398965779890645No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC39800.068.06565
CTTATAG43200.068.0558245
TTATAGG71300.067.987656
GTATAGG80150.067.947876
CGTATAG45750.067.858195
CTATAGG42850.067.713226
CTATACG34350.067.452966
TTATAAC39600.067.437146
CTATACC37850.067.41116
CCTATAC27300.067.307965
CTTATAC45200.067.1352165
GCTATAC43900.067.050385
TTATACC46100.067.039316
GGTATAG67300.066.879915
CGTATAC40650.066.814535
GTTATAG64350.066.791255
CTATAGC40350.066.790856
TTATACG44200.066.595286
CCTATAG23950.066.492965
TATAGGC64400.066.467657
Produced by FastQC (version 0.11.5)