FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_10mM-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_10mM-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784695
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC82350.2175868861295296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG46500.066.685945
GTATAGG85850.066.369366
CTATACG34700.066.366656
TTATAGG88900.066.100156
CTATAGG48200.065.8587956
CCTATAC29250.065.3311545
GGTATAC44750.064.9140245
CGTATAG45400.064.755555
TATAGGG90150.064.484817
CTATAAC38200.064.133996
CGTATAC35200.064.131385
CTATACC32600.064.092956
AGTATAG64200.064.000985
TTATAGC53750.063.9420476
GCTATAC39250.063.57755
GTATAGC52400.063.518876
GCTATAG53950.063.510395
GATATAC40350.063.4923025
CTATAGC42100.063.4301836
TATAGCG52400.063.3184977
Produced by FastQC (version 0.11.5)