FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_10mM-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_10mM-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4470964
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC105270.23545257801225863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG43450.067.503516
TTATAGG104700.067.092326
GTATAGG107150.066.864846
CTATAGG61850.066.4921346
GGTATAC58850.066.372765
CGTATAG58500.066.351055
CTTATAG55150.066.3197565
CTATACC41400.065.942656
CCTATAC36150.065.837415
TATAGGG111300.064.906277
GGTATAG96650.064.8221365
AGTATAG75200.064.601675
GCTATAC49500.064.556175
GTATAGC59950.064.3959356
CGTATAC44200.064.299255
TATAGGC81150.064.09187
TTATAGC66350.064.039796
TTATAAC55300.063.861366
TATACGG70200.063.6686947
CTATAAC46100.063.6231586
Produced by FastQC (version 0.11.5)