FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0mM-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0mM-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332081
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG34000.066.703886
CTATAGG43200.066.5142066
TTATAGG71100.066.404476
GTATAGG80650.066.048866
CTATACC29050.064.817336
GGTATAC44350.064.789485
CTTATAG39100.064.716735
GGTATAG75100.064.6851355
GTTATAG78600.064.342855
CGTATAG45900.064.2028665
CTATAAC32800.064.129176
TTATAGC50950.063.8155566
TTATAAC40100.063.8010836
CCTATAG26900.063.752735
CCTATAC26900.063.752735
TATAGGG72400.063.278497
GCTATAC41150.063.0236245
CGTATAC37050.063.0075575
TATAGGC55050.063.0044677
AGTATAG55450.062.9917955
Produced by FastQC (version 0.11.5)