FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0mM-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0mM-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2667700
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC27710.10387224950331746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG63600.065.9813846
CTATAGG31900.065.719686
TTATAGG57750.065.635046
GGTATAC31750.065.589235
CTATACG25550.065.4781346
CTATACC23350.065.202126
CGTATAG34700.064.9554755
TATAGGG60350.064.895147
CTTATAG31250.064.62275
GGTATAG61150.064.561265
TTATAGC40450.064.028386
CCTATAC20100.063.555945
TATAGTC37250.063.421557
TATAGGC44500.063.313337
GATATAC30550.063.2393155
AGTATAG41200.063.117665
TATACGG41250.063.041167
TATAGCG39250.062.954157
CTATAAC24300.062.9411246
GGCTATA40350.062.6257554
Produced by FastQC (version 0.11.5)