FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0mM-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0mM-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3436541
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG76950.065.177576
CTATAGG35600.064.2966466
GGTATAG72700.064.125445
CTATACG28550.063.7467086
TTATAGG79650.063.495456
CGTATAG36950.063.27365
CTATACC24750.062.6453826
CCTATAC25700.062.508655
CTTATAG40150.062.240525
GTTATAG81200.062.1972055
CTATAAC25600.060.975516
TTATAGC53350.060.9455266
GGTATAC41600.060.828285
CCTATAG24450.060.1216625
TTATAGT102050.059.4698226
GCTATAC35450.059.43485
TATAGGG79450.059.426297
TATAGCG46100.059.2939877
TTTATAG93300.058.5198865
TCTATAG54000.058.4619525
Produced by FastQC (version 0.11.5)