FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0.25mM-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0.25mM-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311272
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC64070.19349059817496117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG30700.066.462476
GGTATAC41900.066.237735
TTATAGG74650.066.011436
CTATAGG42500.065.879056
CCTATAC24250.065.811145
GTATAGG76550.065.5617146
CTATACC29700.065.047256
CTTATAG40350.064.9657755
GGTATAG72100.064.656965
TTATAGC50900.064.564556
GATATAC35150.064.420685
CGTATAG43600.063.8958855
TATAGTC50650.063.708567
TATAGGG78800.063.6897057
CTATAAC32750.063.5846066
AGTATAG54650.063.5283855
CGTATAC34650.063.4311685
TATACGG50800.063.1070757
TATAGGC58200.063.0810367
GCTATAC36300.062.8618625
Produced by FastQC (version 0.11.5)