FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0.25mM-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0.25mM-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3322930
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC78490.23620720267956294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG30950.067.510016
TTATAGG77750.066.621726
CGTATAG45100.066.350485
GTATAGG84100.066.3356256
GGTATAC43500.066.135875
CTATACC29950.065.790936
CTATAGG44600.065.4463966
CCTATAC25250.065.2850955
CTTATAG41450.065.1849445
CTATAAC34250.064.78636
TATAGGG86700.064.6692667
GGTATAG76750.064.662485
TTATAGC50200.064.1414266
GCTATAC37500.064.1180045
TATAGCG52150.064.0919657
TATACGC36250.063.5290537
CGTATAC31650.063.362955
GCTATAA51750.063.234745
CTATAAG38800.063.2325636
TATAGGC61050.063.175757
Produced by FastQC (version 0.11.5)