FastQCFastQC Report
Wed 17 Jul 2019
HNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0.25mM-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKHVBGXB_n01_ZmLeafProtoplast_24h_NH4NO3_0.25mM-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3305348
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGGTGTTACTAATAGGACC45600.13795824221836855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG75700.067.039866
CTATACG32150.066.297536
TTATAGG71600.065.795036
CTATACC29750.065.6459966
CTATAGG43400.065.482816
CGTATAG45800.065.260955
CCTATAC26150.065.180595
GGTATAG71400.064.802875
CTATAAC35100.064.6143346
TATAGGG74600.064.181277
CTTATAG41800.064.137715
GCTATAC39350.064.128565
TTATAGC51750.064.047276
GGTATAC41150.064.0451355
TATAGCG52250.063.836297
GGTATAA64150.063.615495
AGTATAG53500.063.3260775
TATAGGC59950.063.051527
GTATAGA47700.062.6614576
CGCTATA39400.062.5370524
Produced by FastQC (version 0.11.5)