FastQCFastQC Report
Thu 3 Jun 2021
HNKGJDSXY_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKGJDSXY_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11703605964
Sequences flagged as poor quality0
Sequence length10
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGATGCTC126629116510.819666766764705No Hit
CAGGGTTGGC120556692410.300816070775912No Hit
GTAACAGGAA117577676910.046277810588121No Hit
CTCCAAGTTC11474306569.804077986985106No Hit
ATCAGTCTAA11413415269.752050175909357No Hit
GATGAAGATA11109085399.492019317953176No Hit
GAAGTTAGGG10203774578.718487790332787No Hit
AGGTAACACT10151194848.67356169647613No Hit
AAAGGTAGTA9394092118.026664721023582No Hit
AGATCTCGGT3662444663.1293301152359265Illumina Single End PCR Primer 1 (100% over 10bp)
ACCCGTCGAC1520705651.2993479570977122No Hit
CGAAGCGTGG1424380971.2170445368558718No Hit
TCAGCTGTCA1331473831.1376611910000902No Hit
CTTTATTGTC1328656751.1352541721644722No Hit
TGTGCACGGT1041709470.8900756512174729No Hit
AAGCACGTTC885959610.7569971278298241No Hit
TGCTACATTG867182390.7409531666286711No Hit
TTTCAAGCCT692298230.5915255794919038No Hit
ACTTGTGTCA589043520.5033008816358678No Hit
GGGGGGGGGG419160840.3581467466431528No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)