FastQCFastQC Report
Thu 3 Jun 2021
HNKGJDSXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKGJDSXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11703605964
Sequences flagged as poor quality0
Sequence length10
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCCCTGT122280404610.448096507702965No Hit
GTGGATCAAA117545229110.043505348827207No Hit
CGAAGTATAC11278221899.636535888760719No Hit
CACTACGAAA11262016849.62268968610331No Hit
CTCTAGCGAG10773744559.205491523842966No Hit
AAGTGGAGAG10748482659.18390680877506No Hit
TAACGCGTGA10098983908.628950710630741No Hit
GTAACATGCG9983109458.5299432334853No Hit
TCCCAAGGGT9214387317.873118198223031No Hit
GGGGGGGGGG4593524683.9248798140757364No Hit
AGATGAGAAT1483044801.2671691139994021No Hit
CTGTTAGAGG1390770111.1883261571501758No Hit
ATTTACCGCA1358526351.1607758789716547No Hit
TTGTTCATGT1274882371.0893073245301546No Hit
CCAAAGCCGG1024878380.8756945364979821No Hit
TTGTCGTAGA864252700.7384499295844543No Hit
TCAGGAAGGA862800290.7372089359928489No Hit
AAGTGGCGAG716608800.6122974425183749No Hit
AGTCATAATG688303870.5881126484582663No Hit
AGTCCTGCGG612434990.5232874311420213No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)