FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah8921i-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah8921i-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15108600
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2156141.4270945024687927TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC864780.572375997776101TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG286450.037.7143671
CGTATGC120950.037.032344
ACTGACC363700.029.40901233
CAGTCAC368050.029.2885628
CCAGTCA372000.028.92434527
TCGTATG364750.028.8056644
TCCAGTC373750.028.7771426
GCACACG376150.028.72804612
ACGTCTG375950.028.71992116
AGTCACT375900.028.71203829
ACACGTC377150.028.70437214
CACACGT378000.028.61654513
CACGTCT378100.028.60315915
CTCGTAT348700.028.58583643
CACTGAC377100.028.49232932
CTGACCA376000.028.46451634
CGTCTGA380550.028.39010617
GTCACTG380150.028.34474830
ACTCCAG380000.028.34435824
GATCGGA382650.028.3260672
Produced by FastQC (version 0.11.2)