FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah8921a-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah8921a-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18632794
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC347300.18639179931898567TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC67500.028.81102644
GCACACG104500.025.07296411
ACACGTC106950.024.41631713
CACACGT108000.024.24004412
AGATCGG57250.024.0254171
ACGTCTG111550.023.44890215
CGTCTGA111300.023.44227616
CCGATGT111350.023.2341833
CACGTCT113000.023.10907214
CAGTCAC113600.022.87082327
TCCAGTC115750.022.44600925
AGCACAC117800.022.39220810
CACCGAT116350.022.34916731
TATCTCG90750.022.25390839
GTCACCG117050.022.15912629
GATCGGA109150.022.1383381
AGTCACC119400.021.96251928
GAGCACA120400.021.926939
CGATGTA118150.021.8783434
ACCGATG119200.021.8701832
Produced by FastQC (version 0.11.2)