FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah8643i-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah8643i-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15046271
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC153650.10211832553062483TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC40550.022.73169744
GCACACG62650.020.82295211
ACGTCTG64300.020.49389515
ACACGTC63850.020.36269613
CACACGT64500.020.08927512
CGTCTGA67200.019.47853916
CACGTCT67350.019.40249314
CAGTCAC68200.019.03164527
CATCTCG48700.018.97269439
GATCGGA67850.018.6831531
AGCACAC70900.018.6799110
AGTCACC70700.018.63872128
TCCAGTC70950.018.38700525
GAGCACA72950.018.0946649
CCAGTCA72400.017.89721326
AGATCGG43650.017.7474521
CCAGATC73900.017.6232533
AGAGCAC77650.016.9710968
TCGGAAG80350.016.8133663
ACTCCAG78600.016.45748523
Produced by FastQC (version 0.11.2)