FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah8643a-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah8643a-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20716979
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG37650.020.33394612
ACACGTC41400.018.54524214
AGATCGG44300.017.8350471
CACACGT43700.017.66985913
ACGTCTG44950.017.22742716
CACGTCT45550.017.19368715
CGTCTGA45900.016.77501117
GATCGGA48100.016.0571332
CAGTCAC49300.015.79660528
AGCACAC51550.015.23515911
TCCAGTC53600.014.69351526
GTCACCT54450.014.46413830
CCAGTCA54750.014.34470227
GAGCACA55200.014.30795210
AGTCACC55750.013.89009629
AGAGCAC59150.013.1665089
TCGTATG56000.012.885262544
CTCGTAT50750.012.397593543
TCGGAAG64850.012.1130564
ACTCCAG66300.011.94528224
Produced by FastQC (version 0.11.2)