FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah8630i-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah8630i-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14967917
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA745130.49781809987321546TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT230580.1540494913220056TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG115250.031.6207621
GCACACG133400.027.5074912
CAGTCAC133250.027.37335628
GTCACAG134250.027.33332630
ACACGTC134800.027.27076314
CACAGTC134900.027.2505532
TCCAGTC133950.027.09891926
CCAGTCA134400.027.04092227
ACGTCTG135350.026.98115716
CACACGT136600.026.92751913
CACGTCT136200.026.82892415
AGTCACA138300.026.5010829
AGTCAAC138200.026.3610735
ATCTCGT129600.026.22613143
CGTCTGA140300.026.0919417
AGCACAC143150.025.86446611
GAGCACA143000.025.81536710
ACAGTCA143300.025.68387233
ACTCCAG141750.025.60776324
GATCGGA143750.025.4238992
Produced by FastQC (version 0.11.2)