FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah8630a-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah8630a-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18396786
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG36200.015.86143912
AGATCGG37450.015.5734231
ACACGTC38600.015.160214
ACGTCTG39750.014.44488116
CACACGT40500.014.23170313
GATCGGA42150.014.0945872
CACGTCT41950.013.8446715
CGTCTGA41400.013.81604117
CCGTATC42750.013.22536339
CAGTCAC46700.012.71913228
TCCAGTC46750.012.56435626
GTTCCGT45900.012.55738536
TATCTCG39650.012.42840542
GTCACAG47850.011.99966630
AGCACAC49100.011.87339711
CCAGTCA49900.011.85938927
GAGCACA51650.011.54297710
AGTCACA51450.011.5021129
CGTATCT45900.011.07158840
ACTCCAG57000.010.57514524
Produced by FastQC (version 0.11.2)