FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah8038i-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah8038i-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14358709
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC577530.4022158259492549TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG221670.15438017442933064TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC83450.033.08475544
GTCACGC95800.029.48560329
TCACGCC98600.028.7375330
AGTCACG100000.028.35720628
CACGCCA101600.027.84567631
GCACACG103600.027.79651811
ACACGTC104450.027.46500413
TATCTCG103350.027.07616439
CACACGT106300.027.06979612
CGTCTGA106450.026.82498716
ACGTCTG106200.026.80527715
CACGTCT106700.026.72090114
CAGTCAC109000.026.0561527
GATCGGA107100.025.7274231
AGCACAC112300.025.6827210
CCAGTCA111850.025.45123126
GAGCACA113850.025.3910359
TCGTATG109600.025.23104543
TCCAGTC112850.025.22569825
CTCGTAT109750.025.21660642
Produced by FastQC (version 0.11.2)