FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah8038a-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah8038a-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12907127
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20350.013.7292511
CACACGT22150.013.01083212
GTCACAC21900.012.858047529
ACGTCTG22150.012.71287315
CACACAG22200.012.48609831
ACACGTC22750.012.18418913
CGTCTGA23550.011.77028916
CGTATGC22050.011.57329144
CACGTCT24500.011.3138914
GATCGGA22600.011.1989841
TCCAGTC28000.010.37110725
AGCACAC28000.010.21424610
CAGTCAC28150.010.15950227
CCAGTCA30150.09.85040226
CACAGTG29800.09.81848733
GAGCACA29650.09.5716329
AGTCACA31650.09.383594528
AGAGCAC32050.09.0608068
ACACAGT32050.09.0605632
AGATCGG20150.08.9562871
Produced by FastQC (version 0.11.2)