FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah7797k-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah7797k-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13808331
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA344490.24947982489701326TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT219940.15928065455557228TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTT143750.10410381964337326TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG145950.038.602961
GATCGGA201750.027.9641152
GCACACG201500.027.82936512
CACACGT203650.027.58957313
ACACGTC203950.027.52741814
ACGTCTG204650.027.4225116
CACGTCT207550.027.09234615
AGCACAC209100.026.93360511
GAGCACA209700.026.8151610
CGTCTGA210850.026.63702617
CAGTCAC208150.026.61263328
TCCAGTC207650.026.60255226
AGTCACA210750.026.44089329
GTCACAT211550.026.2889130
GAACTCC212900.026.2462122
CCAGTCA211100.026.24073627
TCACATG211900.026.11052331
CACATGT212550.026.06172432
TCGGAAG216550.026.0525464
AGAGCAC216900.025.9351739
Produced by FastQC (version 0.11.2)