FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah7797j-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah7797j-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13065805
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA159500.12207437658835411TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG35450.025.19518336
AGATCGG37600.024.641751
GTCCCGA36850.023.70067837
ACCCGTC39250.022.75590333
TCACCCG39650.022.63730431
GCACACG40700.022.48571212
CCCGTCC40550.022.24337834
CCCGATC36800.022.17858339
CCGTCCC42000.021.4754535
CACCCGT42600.021.1729832
ACACGTC45400.020.49709114
CACACGT45600.020.21421613
ACGTCTG45800.020.12594416
CGTCTGA46450.019.89167217
GATCGGA47050.019.7358252
CACGTCT47300.019.72025115
CGATCTC41650.019.64878541
GATCTCG41750.019.3909542
TCCCGAT44200.019.31155238
GTCACCC46400.019.24933630
Produced by FastQC (version 0.11.2)