FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah6408k-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah6408k-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13188175
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC16500.022.2662233
ACGTCCG16650.022.19775432
AGTCACG20000.019.35961228
GCACACG19650.019.03269811
CCGCACA19900.018.68311536
CACACGT22500.017.11076712
GTCACGT22250.017.10527629
ACACGTC22750.016.82603613
ACGTCTG23250.016.27494215
TCACGTC23350.016.11102930
CACGTCT23650.015.999680514
CACGTCC24100.015.883531
CATCTCG23400.015.32453841
CAGTCAC25700.015.32265127
GTCCGCA25800.014.75164434
TCCAGTC26650.014.2811425
AGCACAC27300.013.94132510
CCAGTCA28550.013.71601326
GAGCACA28200.013.4963889
CTCGTAT29700.013.11084944
Produced by FastQC (version 0.11.2)