FastQCFastQC Report
Wed 30 Aug 2017
HNKF3AFXX_n01_ah6408j-0817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKF3AFXX_n01_ah6408j-0817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21186955
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT331410.1564217227062596TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT69750.024.38065144
GCACACG79500.022.38676811
AGTCACG79050.022.2915728
ACACGTC82750.021.69362613
CGATCTC77000.021.48508640
ACGTCTG82850.021.42846315
GTCACGT83250.021.21980329
CACACGT85800.021.05066912
CACGTCT84550.021.04965214
TCACGTG83850.020.98925230
CACGTGA84550.020.84149731
GATCTCG80700.020.58180441
CGTCTGA88100.020.30133616
GATCGGA83100.020.1802221
AGATCGG51300.019.9912761
AGCACAC93100.019.35341310
CAGTCAC92600.019.0534427
GAGCACA95800.018.8998189
TCCAGTC95650.018.5148825
CCAGTCA96800.018.20401626
Produced by FastQC (version 0.11.2)