FastQCFastQC Report
Tue 6 Oct 2020
HNJW5DRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95796154
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61296986.398689032964726No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC7551520.788290519471168Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC1465190.15294872902726345Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT11892350.023.6653889
AAGAGCG12243800.022.9919878
CGGAAGA12508250.022.4748785
TCGGAAG12594150.022.3204544
GAAGAGC12720400.022.1499847
AGATCGG13044350.021.6403391
GGAAGAG13032000.021.631736
ATCGGAA13040900.021.562913
GATCGGA13136400.021.442712
GCATTCC358500.020.8655433
CACTCTT666800.017.7006952
GATCAAC699650.017.2051241
GATCCAC415000.017.169421
GATCCGC194750.016.3560831
GATCCCG198700.016.2865561
CATTCCT469200.016.2053784
GATCCGG235250.015.6993051
GATCCTG343000.015.5695461
GATCACG287850.015.3260281
GGATCAT799200.015.23954150-54
Produced by FastQC (version 0.11.5)