FastQCFastQC Report
Tue 6 Oct 2020
HNJW5DRXX_n02_DJ65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_n02_DJ65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143873412
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2446040.17001334478673516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC1722900.019.0446621
GATCACG660400.018.9752771
GATCGTG775350.017.5097051
GATCCGC435850.017.4971961
GATCGCG296600.016.806931
GATCCGA948900.016.104261
GATCCAC1067700.015.9638121
GATCCGG532900.015.8901471
GATCCTG818750.015.8105271
GATCCTT1644250.015.6044061
GATCCAG1027550.015.5283411
GATCCCG497450.014.8491341
GATCATG1243550.014.826691
GATCGCA652150.013.3850791
GATCTGC794300.013.3556681
GATCCGT706800.013.2843571
GATCCCA852250.013.1967661
GATCACA1696100.013.0909991
GATCAGC902200.012.9647761
GATCGTC1087550.012.7434471
Produced by FastQC (version 0.11.5)