FastQCFastQC Report
Tue 6 Oct 2020
HNJW5DRXX_n02_DJ64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_n02_DJ64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90538012
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG999280.11037132116397697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC272050.021.0979961
GATCACG419250.020.6654421
GATCAAC1078250.020.5322321
GATCCAC656750.018.9707131
GATCCGG335950.018.7481561
GATCGTG475750.018.424751
GATCGCG203350.018.2700271
GATCCGA586650.018.1329421
GATCCCG311900.017.890581
GATCCTG523050.017.6187041
GATCCAG638100.017.2622131
GATCCTT1037400.017.0250241
GATCCGT457300.016.3436641
GATCCCA515500.015.7793951
GATCATG788600.015.0351821
GATCTGC478600.014.9491881
GATCCTC720600.014.79247951
GATCGTC635300.014.4599991
GATCGCC274700.014.3434831
GATCGCA396150.014.3421921
Produced by FastQC (version 0.11.5)