FastQCFastQC Report
Tue 6 Oct 2020
HNJW5DRXX_n02_DJ63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_n02_DJ63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122281020
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1393650.1139710807122806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGC406000.026.5230731
GATCACG615900.026.1552431
GATCCGG486900.024.962671
GATCAAC1594200.023.5686781
GATCCAC996500.023.3891371
GATCGCG282100.023.278851
GATCCGA880050.023.0127491
GATCCTG783950.022.4645331
GATCGTG717750.022.1708011
GATCCAG947400.021.9588851
GATCCCG458450.021.9375041
GATCTGC759900.020.79781
GATCCTT1583600.020.0103031
GATCGCA601750.019.3662451
GATCCGT664850.019.0234681
GATCATG1163400.018.936021
GATCAGC838350.018.799681
GATCGCC430600.018.7558671
GATCCCA776950.018.5388771
GATCCCT542750.017.5408251
Produced by FastQC (version 0.11.5)