FastQCFastQC Report
Tue 6 Oct 2020
HNJW5DRXX_n02_DJ62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_n02_DJ62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108603678
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2265570.20860895705576382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG540300.024.2544981
GATCAAC1398250.022.1383651
GATCCGC349500.021.9243261
GATCCGG428800.021.0003491
GATCCAC851100.020.8197061
GATCGCG249100.020.5655571
GATCCGA745500.019.8268131
GATCCTG668600.019.7195991
GATCGTG619900.019.5363861
GATCCAG812500.019.3975091
GATCCCG393650.019.2441941
GATCCTT1362850.018.4433291
GATCCCA657800.017.8923131
GATCATG1027050.017.5708891
GATCTGC619300.017.0596371
GATCGCA518750.016.435741
GATCCGT573200.016.3555761
GATCAGC720700.016.08911
GATCACA1374550.015.7893911
GATCCCT470500.015.3606361
Produced by FastQC (version 0.11.5)