FastQCFastQC Report
Tue 6 Oct 2020
HNJW5DRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95796154
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCG14609961.5251092439473093Illumina Paired End PCR Primer 2 (100% over 50bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGT3864870.40344730332284534Illumina Paired End PCR Primer 2 (100% over 50bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGGGGGGGGGGGGGGGGGG3077000.3212028741780176Illumina Single End Adapter 1 (96% over 25bp)
AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCGCGTATGCCG2473190.25817216002220716Illumina Paired End PCR Primer 2 (98% over 50bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAGGGGGGGGGGGGGGGGGGGG2310320.24117043362722057Illumina Single End Adapter 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGTCTATCTCGTAT979780.10227759248038289TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC449800.030.336223
GGTCGGA277650.029.9463671
AGAGCAC4086750.028.8290528
AAGAGCG10180750.028.0518578
AGAGCGG10226500.027.9894319
GAGCACA4559700.026.0565369
AAGAGCA4865150.024.3271587
CATTCCT583950.022.1363374
AGATCGG13330400.021.3097861
GATCACG294400.021.021731
GATCCGC188850.020.4386351
CGGAAGA14334450.019.9432455
TCGGAAG14436750.019.7874574
GAAGAGC14538450.019.704827
GGAAGAG14729900.019.4491776
GATCGGA14815250.019.1948432
ATCGGAA14868300.019.1930083
GATCCAG429800.018.9908031
GATCCTG341600.018.902941
GATCAAC724350.018.900821
Produced by FastQC (version 0.11.5)