FastQCFastQC Report
Tue 6 Oct 2020
HNJW5DRXX_n01_DJ65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJW5DRXX_n01_DJ65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143873412
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCGTAT4388080.3049958945854429TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAAC1722700.019.809771
GATCACG648150.019.7262481
GATCGCG277950.018.4050311
GATCGTG737000.018.322851
GATCCGC432700.017.9604281
GATCCGG508200.016.8485091
GATCCAC1046000.016.4480341
GATCCGA918900.016.1408631
GATCCTG816800.015.7864981
GATCCTT1666400.015.2362751
GATCCAG999400.015.1965151
GATCCCG500100.015.1480871
GATCATG1229800.014.7450381
GATCCCA860450.013.6194951
GATCCGT711050.013.552271
GATCGTC1047200.013.4426911
GATCCCT571100.013.39191151
GATCACA1705250.013.1615171
GATCGCA646400.013.0554691
GATCAGC900050.012.9154631
Produced by FastQC (version 0.11.5)